Fermbox Bio, a synthetic biology research and manufacturing company, announced the launch of EN3ZYME, a solution designed to enhance both the efficiency and cost-effectiveness of transforming pre-treated agriculture-based residues into fermentable, cellulosic sugars. These sugars pave the way to produce second-generation (2G) ethanol and serve as an alternative carbon source in precision fermentation to produce bio-ingredients and biomaterials, such as colors, dyes, flavors, fragrances, lipids, and novel food proteins, fostering a circular economy.
EN3ZYME directly addresses the food vs. fuel challenge associated with first-generation (1G) ethanol by hydrolyzing agricultural residues into a viable alternative carbon source. EN3ZYME is designed to hydrolyze diverse feedstock residues such as bagasse, cotton stalks, corn stover, and other cellulose-rich materials. The technology and its applications support sustainable processes and resource optimization, aligning with global environmental goals.
“Our vision with EN3ZYME was to tackle the twin challenge of energy sustainability and climate change head-on,” said Subramani Ramachandrappa, founder of Fermbox Bio. “Our biomanufacturing model is designed to offer tailored solutions for each plant, whether onsite or offsite, for the production and supply of our enzymes to our customers. This flexibility caters to both large- and small-scale operations, streamlining processes, reducing inventory, and boosting efficiency. It adapts to fluctuating demands and maximizes resource utilization.”
EN3ZYME technology exemplifies Fermbox Bio’s global collaborative model for product development and manufacturing. Co-developed with Dyadic International Inc. USA, and co-manufactured with BBGI Public Limited Company Thailand, EN3ZYME production will be co-located at an existing 1G ethanol plant. This approach leverages global synergies, resulting in significant efficiencies in capital invested and operational costs.
While primarily aimed at the ethanol industry, EN3ZYME’s applications extend beyond 2G ethanol production. According to Fermbox Bio, it provides a cost-efficient, alternative carbon feedstock for developing novel proteins and biomaterials via precision fermentation, reducing reliance on conventional sugar sources that compete with food production.